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KMID : 0379520040200020101
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한국독성학회지
2004 Volume.20 No. 2 p.101 ~ p.108
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Performance Evaluation of PCR Kits for Detecting Genetically Modified Crop Ingredients
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Yun Si-On
Jeong Soon-Chun
Yoon Won-Kee
Park Sang-Kyu
Moon Jae-Sun
Lee Joung-Hyun
Kim Hwan-Mook
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Abstract
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The different social reflections about the benefits and the potential risks of genetically modified (GM) crops have evolved with .different reactions in different countries. Many countries including Korea are working toward setting down new guidelines. Korea requires companies to label all food that contains more than 3% GM ingredients. One of the rapid and convenient detection methods of GM ingredients is amplification of the introduced DNAs by polymerase chain reaction (PCR). Many PCR kits for this purpose are commercially available. The objective of this study was to evaluate performance of commercialized GM crop detection kits. The results showed that 6 out of 15 kits tested did not meet the requirements even purposed by the manufacturers themselves in terms of stability, reproducibility, and detection limits, suggesting a potential quality control problem in their design stage or production line. The evaluation also suggests that, although the duplex and triplex detection kits allowed unambiguous detection in a single PCR reaction, the monoplex detection kits were the most sensitive to the detection of GM ingredients. The detection limits also differ between soybean and corn. Results from this study will be useful in the development of sound qualitative tracking systems of GM ingredients for monitoring throughout the cultivation of GM crops, their trans-boundary movement, and food production using GM grains as well as for complying with government guidelines associated with GM crops.
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KEYWORD
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Corn, Genetically modified crop, PCR detection kit, Soybean
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